Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PODXL / Podocalyxin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100542-100
Catalog Number: LS-G100542
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PODXL (NM_005397), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GCTM-2 antigen | gp200 | PCLP | PCLP-1 | PCLP1 | Podocalyxin-like protein 1 | Podocalyxin | PODXL | Podocalyxin-like | TRA-1-60 | TRA-1-81
- Origin Species
- Human
- NCBI GenBank
- NM_005397
- SwissProt
- O00592
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.39 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRCALALSAL LLLLSTPPLL PSSPSPSPSP SQNATQTTTD SSNKTAPTPA SSVTIMATDT61AQQSTVPTSK ANEILASVKA TTLGVSSDSP GTTTLAQQVS GPVNTTVARG GGSGNPTTTI121ESPKSTKSAD TTTVATSTAT AKPNTTSSQN GAEDTTNSGG KSSHSVTTDL TSTKAEHLTT181PHPTSPLSPR QPTSTHPVAT PTSSGHDHLM KISSSSSTVA IPGYTFTSPG MTTTLPSSVI241SQRTQQTSSQ MPASSTAPSS QETVQPTSPA TALRTPTLPE TMSSSPTAAS TTHRYPKTPS301PTVAHESNWA KCEDLETQTQ SEKQLVLNLT GNTLCAGGAS DEKLISLICR AVKATFNPAQ361DKCGIRLASV PGSQTVVVKE ITIHTKLPAK DVYERLKDKW DELKEAGVSD MKLGDQGPPE421EAEDRFSMPL IITIVCMASF LLLVAALYGC CHQRLSQRKD QQRLTEELQT VENGYHDNPT481LEVMETSSEM QEKKVVSLNG ELGDSWIVPL DNLTKDDLDE EEDTHLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
526 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.