PODXL / Podocalyxin Antibody (clone PODXL/2185)
Catalog Number: LS-C782499
2 sizes / from $379.00
Antibody: Mouse Monoclonal
Applications: Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PODXL / Podocalyxin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G81997-20
Catalog Number: LS-G81997
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PODXL (NM_005397) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GCTM-2 antigen | gp200 | PCLP | PCLP-1 | PCLP1 | Podocalyxin-like protein 1 | Podocalyxin | PODXL | Podocalyxin-like | TRA-1-60 | TRA-1-81
- Origin Species
- Human
- NCBI GenBank
- NM_005397
- SwissProt
- O00592
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.39 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRCALALSAL LLLLSTPPLL PSSPSPSPSP SQNATQTTTD SSNKTAPTPA SSVTIMATDT61AQQSTVPTSK ANEILASVKA TTLGVSSDSP GTTTLAQQVS GPVNTTVARG GGSGNPTTTI121ESPKSTKSAD TTTVATSTAT AKPNTTSSQN GAEDTTNSGG KSSHSVTTDL TSTKAEHLTT181PHPTSPLSPR QPTSTHPVAT PTSSGHDHLM KISSSSSTVA IPGYTFTSPG MTTTLPSSVI241SQRTQQTSSQ MPASSTAPSS QETVQPTSPA TALRTPTLPE TMSSSPTAAS TTHRYPKTPS301PTVAHESNWA KCEDLETQTQ SEKQLVLNLT GNTLCAGGAS DEKLISLICR AVKATFNPAQ361DKCGIRLASV PGSQTVVVKE ITIHTKLPAK DVYERLKDKW DELKEAGVSD MKLGDQGPPE421EAEDRFSMPL IITIVCMASF LLLVAALYGC CHQRLSQRKD QQRLTEELQT VENGYHDNPT481LEVMETSSEM QEKKVVSLNG ELGDSWIVPL DNLTKDDLDE EEDTHLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
526 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
