RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NUP50 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105730-100
Catalog Number: LS-G105730
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NUP50 (NM_153645), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 50 kDa nucleoporin | Nucleoporin 50kD | NUP50 | Nucleoporin 50kDa | NPAP60 | NPAP60L | Nucleoporin Nup50
- Origin Species
- Human
- NCBI GenBank
- NM_007172
- SwissProt
- Q9UKX7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASEEVLKNR AIKKAKRRNV GFESDTGGAF KGFKGLVVPS GGGRFSGFGS GAGGKPLEGL61SNGNNITSAP PFASAKAAAD PKVAFGSLAA NGPTTLVDKV SNPKTNGDSQ QPSSSGLASS121KACVGNAYHK QLAALNCSVR DWIVKHVNTN PLCDLTPIFK DYEKYLANIE QQHGNSGRNS181ESESNKVAAE TQSPSLFGST KLQQESTFLF HGNKTEDTPD KKMEVASEKK TDPSSLGATS241ASFNFGKKVD SSVLGSLSSV PLTGFSFSPG NSSLFGKDTT QSKPVSSPFP TKPLEGQAEG301DSGECKGGDE EENDEPPKVV VTEVKEEDAF YSKKCKLFYK KDNEFKEKGI GTLHLKPTAN361QKTQLLVRAD TNLGNILLNV LIPPNMPCTR TGKNNVLIVC VPNPPIDEKN ATMPVTMLIR421VKTSEDADEL HKILLEKKDATRTRPLEQKLISEEDLAANDILDYKDDDDKV
440 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
