NUP50 Antibody
Catalog Number: LS-C405213
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NUP50 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G88407-20
Catalog Number: LS-G88407
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NUP50 (NM_153645) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 50 kDa nucleoporin | Nucleoporin 50kD | NUP50 | Nucleoporin 50kDa | NPAP60 | NPAP60L | Nucleoporin Nup50
- Origin Species
- Human
- NCBI GenBank
- NM_007172
- SwissProt
- Q9UKX7
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASEEVLKNR AIKKAKRRNV GFESDTGGAF KGFKGLVVPS GGGRFSGFGS GAGGKPLEGL61SNGNNITSAP PFASAKAAAD PKVAFGSLAA NGPTTLVDKV SNPKTNGDSQ QPSSSGLASS121KACVGNAYHK QLAALNCSVR DWIVKHVNTN PLCDLTPIFK DYEKYLANIE QQHGNSGRNS181ESESNKVAAE TQSPSLFGST KLQQESTFLF HGNKTEDTPD KKMEVASEKK TDPSSLGATS241ASFNFGKKVD SSVLGSLSSV PLTGFSFSPG NSSLFGKDTT QSKPVSSPFP TKPLEGQAEG301DSGECKGGDE EENDEPPKVV VTEVKEEDAF YSKKCKLFYK KDNEFKEKGI GTLHLKPTAN361QKTQLLVRAD TNLGNILLNV LIPPNMPCTR TGKNNVLIVC VPNPPIDEKN ATMPVTMLIR421VKTSEDADEL HKILLEKKDATRTRPLEQKLISEEDLAANDILDYKDDDDKV
440 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
