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NUMB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NUMB Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NUMB antibodies →
$725.00each
Catalog Number: LS-G122142-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NUMB (NM_001005744), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14_5527 | NUMB | Numb (Drosophila) homolog | S171 | Protein numb homolog | C14orf41 | H-Numb | Numb homolog (Drosophila) | Protein S171
Origin Species
Human
NCBI GenBank
NM_003744
SwissProt
P49757

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNKLRQSFRR KKDVYVPEAS RPHQWQTDEE GVRTGKCSFP VKYLGHVEVD ESRGMHICED
61AVKRLKAERK FFKGFFGKTG KKAVKAVLWV SADGLRVVDE KTKDLIVDQT IEKVSFCAPD
121RNFDRAFSYI CRDGTTRRWI CHCFMAVKDT GERLSHAVGC AFAACLERKQ KREKECGVTA
181TFDASRTTFT REGSFRVTTA TEQAEREEIM KQMQDAKKAE TDKIVVGSSV APGNTAPSPS
241SPTSPTSDAT TSLEMNNPHA IPRRHAPIEQ LARQGSFRGF PALSQKMSPF KRQLSLRINE
301LPSTMQRKTD FPIKNAVPEV EGEAESISSL CSQITNAFST PEDPFSSAPM TKPVTVVAPQ
361SPTFQGTEWG QSSGAASPGL FQAGHRRTPS EADRWLEEVS KSVRAQQPQA SAAPLQPVLQ
421PPPPTAISQP ASPFQGNAFL TSQPVPVGVV PALQPAFVPA QSYPVANGMP YPAPNVPVVG
481ITPSQMVANV FGTAGHPQAA HPHQSPSLVR QQTFPHYEAS SATTSPFFKP PAQHLNGSAA
541FNGVDDGRLA SADRHTEVPT GTCPVDPFEA QWAALENKSK QRTNPSPTNP FSSDLQKTFE
601IEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

603 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.