NUMB Antibody
Catalog Number: LS-C410886
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NUMB Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G76728-20
Catalog Number: LS-G76728
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NUMB (NM_003744) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C14_5527 | NUMB | Numb (Drosophila) homolog | S171 | Protein numb homolog | C14orf41 | H-Numb | Numb homolog (Drosophila) | Protein S171
- Origin Species
- Human
- NCBI GenBank
- NM_003744
- SwissProt
- P49757
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNKLRQSFRR KKDVYVPEAS RPHQWQTDEE GVRTGKCSFP VKYLGHVEVD ESRGMHICED61AVKRLKATGK KAVKAVLWVS ADGLRVVDEK TKDLIVDQTI EKVSFCAPDR NFDRAFSYIC121RDGTTRRWIC HCFMAVKDTG ERLSHAVGCA FAACLERKQK REKECGVTAT FDASRTTFTR181EGSFRVTTAT EQAEREEIMK QMQDAKKAET DKIVVGSSVA PGNTAPSPSS PTSPTSDATT241SLEMNNPHAI PRRHAPIEQL ARQGSFRGFP ALSQKMSPFK RQLSLRINEL PSTMQRKTDF301PIKNAVPEVE GEAESISSLC SQITNAFSTP EDPFSSAPMT KPVTVVAPQS PTFQANGTDS361AFHVLAKPAH TALAPVAMPV RETNPWAHAP DAANKEIAAT CSGTEWGQSS GAASPGLFQA421GHRRTPSEAD RWLEEVSKSV RAQQPQASAA PLQPVLQPPP PTAISQPASP FQGNAFLTSQ481PVPVGVVPAL QPAFVPAQSY PVANGMPYPA PNVPVVGITP SQMVANVFGT AGHPQAAHPH541QSPSLVRQQT FPHYEASSAT TSPFFKPPAQ HLNGSAAFNG VDDGRLASAD RHTEVPTGTC601PVDPFEAQWA ALENKSKQRT NPSPTNPFSS DLQKTFEIELTRTRPLEQKLISEEDLAANDILDYKDDDDKV
640 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
