Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NFKBIZ / IKBZ Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122088-100
Catalog Number: LS-G122088
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IKB zeta(NFKBIZ) (NM_001005474), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IkB-zeta | NFKBIZ | IkappaBzeta | IKBZ | MAIL | I-kappa-B-zeta | Ikappa B-zeta variant 3 | IkappaB-zeta | INAP | NF-kappa-B inhibitor zeta
- Origin Species
- Human
- NCBI GenBank
- NM_031419
- SwissProt
- Q9BYH8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGVGRQQRGP FQGVRVKNSV KELLLHIRSH KQKASGQAVD DFKTQGVNIE QFRELKNTVS61YSGKRKGPDS LSDGPACKRP ALLHSQFLTP PQTPTPGESM EDVHLNEPKQ ESSADLLQNI121INIKNECSPV SLNTVQVSWL NPVVVPQSSP AEQCQDFHGG QVFSPPQKCQ PFQVRGSQQM181IDQASLYQYS PQNQHVEQQP HYTHKPTLEY SPFPIPPQSP AYEPNLFDGP ESQFCPNQSL241VSLLGDQRES ENIANPMQTS SSVQQQNDAH LHSFSMMPSS ACEAMVGHEM ASDSSNTSLP301FSNMGNPMNT TQLGKSLFQW QVEQEESKLA NISQDQFLSK DADGDTFLHI AVAQGRRALS361YVLARKMNAL HMLDIKEHNG QSAFQVAVAA NQHLIVQDLV NIGAQVNTTD CWGRTPLHVC421AEKGHSQVLQ AIQKGAVGSN QFVDLEATNY DGLTPLHCAV IAHNAVVHEL QRNQQPHSPE481VQELLLKNKS LVDTIKCLIQ MGAAVEAKDR KSGRTALHLA AEEANLELIR LFLELPSCLS541FVNAKAYNGN TALHVAASLQ YRLTQLDAVR LLMRKGADPS TRNLENEQPV HLVPDGPVGE601QIRRILKGKS IQQRAPPYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
618 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.