Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NFKBIZ / IKBZ Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109224-100
Catalog Number: LS-G109224
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IKB zeta(NFKBIZ) (NM_031419), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IkB-zeta | NFKBIZ | IkappaBzeta | IKBZ | MAIL | I-kappa-B-zeta | Ikappa B-zeta variant 3 | IkappaB-zeta | INAP | NF-kappa-B inhibitor zeta
- Origin Species
- Human
- NCBI GenBank
- NM_031419
- SwissProt
- Q9BYH8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIVDKLLDDS RGGEGLRDAA GGCGLMTSPL NLSYFYGASP PAAAPGACDA SCSVLGPSAP61GSPGSDSSDF SSASSVSSCG AVESRSRGGA RAERQPVEPH MGVGRQQRGP FQGVRVKNSV121KELLLHIRSH KQKASGQAVD DFKTQGVNIE QFRELKNTVS YSGKRKGPDS LSDGPACKRP181ALLHSQFLTP PQTPTPGESM EDVHLNEPKQ ESSADLLQNI INIKNECSPV SLNTVQVSWL241NPVVVPQSSP AEQCQDFHGG QVFSPPQKCQ PFQVRGSQQM IDQASLYQYS PQNQHVEQQP301HYTHKPTLEY SPFPIPPQSP AYEPNLFDGP ESQFCPNQSL VSLLGDQRES ENIANPMQTS361SSVQQQNDAH LHSFSMMPSS ACEAMVGHEM ASDSSNTSLP FSNMGNPMNT TQLGKSLFQW421QVEQEESKLA NISQDQFLSK DADGDTFLHI AVAQGRRALS YVLARKMNAL HMLDIKEHNG481QSAFQVAVAA NQHLIVQDLV NIGAQVNTTD CWGRTPLHVC AEKGHSQVLQ AIQKGAVGSN541QFVDLEATNY DGLTPLHCAV IAHNAVVHEL QRNQQPHSPE VQELLLKNKS LVDTIKCLIQ601MGAAVEAKDR KSGRTALHLA AEEANLELIR LFLELPSCLS FVNAKAYNGN TALHVAASLQ661YRLTQLDAVR LLMRKGADPS TRNLENEQPV HLVPDGPVGE QIRRILKGKS IQQRAPPYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
718 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.