Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.

Mouse TNF Alpha Protein (Recombinant)

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Expression System	Escherichia coli (Prokaryotic)
Format:	Lyophilized

$324.00each

Catalog Number: LS-G49419-20

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Overview

Description: TNF Alpha Protein LS-G49419 is a Recombinant Mouse TNF Alpha produced in E. coli Leu80-Leu235. It is biologically active. For Research Use Only
Category: Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names: APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | macrophage-derived | monocyte-derived | TNFSF2
Origin Species: Mouse
NCBI GenBank: NM_000594
SwissProt: P01375

Protein

Expression System: Escherichia coli (Prokaryotic)
Molecular Weight: The recombinant mouse TNF-a consists of 157 amino acids and migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.
Protein Type: Recombinant
Amino Acid Sequence

Format

Purity: >98%
Conjugate/Label: Unconjugated
Endotoxin Level: Not Tested
Format: Lyophilized
Formulation: Lyophilized from sterile 50mM Tris 0.2M NaCl, pH 7.2, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Purity Measurement Method: SDS-PAGE (Coomassie Stain)