Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAP4K3 / GLK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G100083-100
Catalog Number: LS-G100083
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP4K3 (NM_003618), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MEK kinase kinase 3 | RAB8IPL1 | MAP4K3 | MEKKK 3 | MEKKK3 | GCK-like kinase | GLK | MAPKKKK3
- Origin Species
- Human
- NCBI GenBank
- NM_003618
- SwissProt
- Q8IVH8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 101.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNPGFDLSRR NPQEDFELIQ RIGSGTYGDV YKARNVNTGE LAAIKVIKLE PGEDFAVVQQ61EIIMMKDCKH PNIVAYFGSY LRRDKLWICM EFCGGGSLQD IYHVTGPLSE LQIAYVSRET121LQGLYYLHSK GKMHRDIKGA NILLTDNGHV KLADFGVSAQ ITATIAKRKS FIGTPYWMAP181EVAAVERKGG YNQLCDLWAV GITAIELAEL QPPMFDLHPM RALFLMTKSN FQPPKLKDKM241KWSNSFHHFV KMALTKNPKK RPTAEKLLQH PFVTQHLTRS LAIELLDKVN NPDHSTYHDF301DDDDPEPLVA VPHRIHSTSR NVREEKTRSE ITFGQVKFDP PLRKETEPHH ELPDSDGFLD361SSEEIYYTAR SNLDLQLEYG QGHQGGYFLG ANKSLLKSVE EELHQRGHVA HLEDDEGDDD421ESKHSTLKAK IPPPLPPKPK SIFIPQEMHS TEDENQGTIK RCPMSGSPAK PSQVPPRPPP481PRLPPHKPVA LGNGMSSFQL NGERDGSLCQ QQNEHRGTNL SRKEKKDVPK PISNGLPPTP541KVHMGACFSK VFNGCPLKIH CASSWINPDT RDQYLIFGAE EGIYTLNLNE LHETSMEQLF601PRRCTWLYVM NNCLLSISGK ASQLYSHNLP GLFDYARQMQ KLPVAIPAHK LPDRILPRKF661SVSAKIPETK WCQKCCVVRN PYTGHKYLCG ALQTSIVLLE WVEPMQKFML IKHIDFPIPC721PLRMFEMLVV PEQEYPLVCV GVSRGRDFNQ VVRFETVNPN STSSWFTESD TPQTNVTHVT781QLERDTILVC LDCCIKIVNL QGRLKSSRKL SSELTFDFQI ESIVCLQDSV LAFWKHGMQG841RSFRSNEVTQ EISDSTRIFR LLGSDRVVVL ESRPTDNPTA NSNLYILAGH ENSYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
894 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.