Western blot of anti-GLK antibody in HeLa cell lysate. Dilution for anti-GLK was 1:100; dilution for secondary goat anti-rabbit-HRP was 1:7000. A chemiluminescent kit was used for development of the Western blot. Data and protocol courtesy of Dr. Richard Lu, Partners HealthCare System at Harvard University.
MAP4K3 / GLK Antibody (aa423-453)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C99818-400
Catalog Number: LS-C99818
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- Description
- GLK antibody LS-C99818 is an unconjugated rabbit polyclonal antibody to human GLK (MAP4K3) (aa423-453). Validated for IHC and WB.
- Application
- IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- MEK kinase kinase 3 | RAB8IPL1 | MAP4K3 | MEKKK 3 | MEKKK3 | GCK-like kinase | GLK | MAPKKKK3
- Recommended Dilution: IHC-P
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q8IVH8
- NCBI GenBank
- NM_003618 | NP_003609.2
- SwissProt
- Q8IVH8
Target
- Target
- Human MAP4K3 / GLK
- Antigen Species
- Human
- Epitope
- This GLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-453 amino acids from the C-terminal region of human GLK.
- Gene Name
- MAP4K3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Ammonium Sulfate Precipitation
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS