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KARS Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KARS Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KARS antibodies →
$504.00each
Catalog Number: LS-G125002-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Lysyl tRNA synthetase(KARS) (NM_001130089), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CMTRIB | KARS1 | KARS2 | KRS | Lysine--tRNA ligase | LysRS | KARS | KIAA0070 | Lysine tRNA ligase | Lysyl-tRNA synthetase
Origin Species
Human
NCBI GenBank
NM_005548
SwissProt
Q15046

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLTQAAVRLV RGSLRKTSWA EWGHRELRLG QLAPFTAPHK DKSFSDQRSE LKRRLKAEKK
61VAEKEAKQKE LSEKQLSQAT AAATNHTTDN GVGPEEESVD PNQYYKIRSQ AIHQLKVNGE
121DPYPHKFHVD ISLTDFIQKY SHLQPGDHLT DITLKVAGRI HAKRASGGKL IFYDLRGEGV
181KLQVMANSRN YKSEEEFIHI NNKLRRGDII GVQGNPGKTK KGELSIIPYE ITLLSPCLHM
241LPHLHFGLKD KETRYRQRYL DLILNDFVRQ KFIIRSKIIT YIRSFLDELG FLEIETPMMN
301IIPGGAVAKP FITYHNELDM NLYMRIAPEL YHKMLVVGGI DRVYEIGRQF RNEGIDLTHN
361PEFTTCEFYM AYADYHDLME ITEKMVSGMV KHITGSYKVT YHPDGPEGQA YDVDFTPPFR
421RINMVEELEK ALGMKLPETN LFETEETRKI LDDICVAKAV ECPPPRTTAR LLDKLVGEFL
481EVTCINPTFI CDHPQIMSPL AKWHRSKEGL TERFELFVMK KEICNAYTEL NDPMRQRQLF
541EEQAKAKAAG DDEAMFIDEN FCTALEYGLP PTAGWGMGID RVAMFLTDSN NIKEVLLFPA
601MKPEDKKENV ATTDTLESTT VGTSV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

625 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.