KARS Antibody
Catalog Number: LS-C405098
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human KARS Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G77172-20
Catalog Number: LS-G77172
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Lysyl tRNA synthetase(KARS) (NM_001130089) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CMTRIB | KARS1 | KARS2 | KRS | Lysine--tRNA ligase | LysRS | KARS | KIAA0070 | Lysine tRNA ligase | Lysyl-tRNA synthetase
- Origin Species
- Human
- NCBI GenBank
- NM_005548
- SwissProt
- Q15046
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLTQAAVRLV RGSLRKTSWA EWGHRELRLG QLAPFTAPHK DKSFSDQRSE LKRRLKAEKK61VAEKEAKQKE LSEKQLSQAT AAATNHTTDN GVGPEEESVD PNQYYKIRSQ AIHQLKVNGE121DPYPHKFHVD ISLTDFIQKY SHLQPGDHLT DITLKVAGRI HAKRASGGKL IFYDLRGEGV181KLQVMANSRN YKSEEEFIHI NNKLRRGDII GVQGNPGKTK KGELSIIPYE ITLLSPCLHM241LPHLHFGLKD KETRYRQRYL DLILNDFVRQ KFIIRSKIIT YIRSFLDELG FLEIETPMMN301IIPGGAVAKP FITYHNELDM NLYMRIAPEL YHKMLVVGGI DRVYEIGRQF RNEGIDLTHN361PEFTTCEFYM AYADYHDLME ITEKMVSGMV KHITGSYKVT YHPDGPEGQA YDVDFTPPFR421RINMVEELEK ALGMKLPETN LFETEETRKI LDDICVAKAV ECPPPRTTAR LLDKLVGEFL481EVTCINPTFI CDHPQIMSPL AKWHRSKEGL TERFELFVMK KEICNAYTEL NDPMRQRQLF541EEQAKAKAAG DDEAMFIDEN FCTALEYGLP PTAGWGMGID RVAMFLTDSN NIKEVLLFPA601MKPEDKKENV ATTDTLESTT VGTSVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
625 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
