TRIM50 / E3 Ubiquitin Ligase Antibody (aa360-409)
Catalog Number: LS-C109918-100
100 µL (0.5 mg/mL) / $433.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human TRIM50 / E3 Ubiquitin Ligase Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G94029-20
Catalog Number: LS-G94029
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TRIM50 (NM_178125) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- E3 ubiquitin ligase | TRIM50A | Tripartite motif-containing 50 | Tripartite motif containing 50 | Tripartite motif protein 50 | TRIM50
- Origin Species
- Human
- SwissProt
- Q86XT4
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAWQVSLPEL EDRLQCPICL EVFKEPLMLQ CGHSYCKGCL VSLSCHLDAE LRCPVCRQAV61DGSSSLPNVS LARVIEALRL PGDPEPKVCV HHRNPLSLFC EKDQELICGL CGLLGSHQHH121PVTPVSTVYS RMKEELAALI SELKQEQKKV DELIAKLVNN RTRIVNESDV FSWVIRREFQ181ELHHLVDEEK ARCLEGIGGH TRGLVASLDM QLEQAQGTRE RLAQAECVLE QFGNEDHHKF241IRKFHSMASR AEMPQARPLE GAFSPISFKP GLHQADIKLT VWKRLFRKVL PAPEPLKLDP301ATAHPLLELS KGNTVVQCGL LAQRRASQPE RFDYSTCVLA SRGFSCGRHY WEVVVGSKSD361WRLGVIKGTA SRKGKLNRSP EHGVWLIGLK EGRVYEAFAC PRVPLPVAGH PHRIGLYLHY421EQGELTFFDA DRPDDLRPLY TFQADFQGKL YPILDTCWHE RGSNSLPMVL PPPSGPGPLS481PEQPTKLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
487 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
