Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRIM50 / E3 Ubiquitin Ligase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104781-100
Catalog Number: LS-G104781
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRIM50 (NM_178125), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- E3 ubiquitin ligase | TRIM50A | Tripartite motif-containing 50 | Tripartite motif containing 50 | Tripartite motif protein 50 | TRIM50
- Origin Species
- Human
- SwissProt
- Q86XT4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAWQVSLPEL EDRLQCPICL EVFKEPLMLQ CGHSYCKGCL VSLSCHLDAE LRCPVCRQAV61DGSSSLPNVS LARVIEALRL PGDPEPKVCV HHRNPLSLFC EKDQELICGL CGLLGSHQHH121PVTPVSTVYS RMKEELAALI SELKQEQKKV DELIAKLVNN RTRIVNESDV FSWVIRREFQ181ELHHLVDEEK ARCLEGIGGH TRGLVASLDM QLEQAQGTRE RLAQAECVLE QFGNEDHHKF241IRKFHSMASR AEMPQARPLE GAFSPISFKP GLHQADIKLT VWKRLFRKVL PAPEPLKLDP301ATAHPLLELS KGNTVVQCGL LAQRRASQPE RFDYSTCVLA SRGFSCGRHY WEVVVGSKSD361WRLGVIKGTA SRKGKLNRSP EHGVWLIGLK EGRVYEAFAC PRVPLPVAGH PHRIGLYLHY421EQGELTFFDA DRPDDLRPLY TFQADFQGKL YPILDTCWHE RGSNSLPMVL PPPSGPGPLS481PEQPTKLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
487 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.