Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human HFip1 / FIP1L1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G91996-20
Catalog Number: LS-G91996
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FIP1L1 (NM_001134938) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Factor interacting with PAP | FIP1-like 1 protein | FIP1 | FIP1 like 1 (S. cerevisiae) | FIP1L1 | HFip1 | RHE
- Origin Species
- Human
- NCBI GenBank
- NM_030917
- SwissProt
- Q6UN15
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSAGEVERLV SELSGGTGGD EEEEWLYGDE NEVERPEEEN ASANPPSGIE DETAENGVPK61PKVTETEDDS DSDSDDDEDD VHVTIGDIKT GAPQYGSYGT APVNLNIKTG GRVYGTTGTK121VKGVDLDAPG SINGVPLLEV DLDSFEDKPW RKPGADLSDY FNYGFNEDTW KAYCEKQKRI181RMGLEVIPVT STTNKITVQQ GRTGNSEKET ALPSTKAEFT SPPSLFKTGL PPSRRLPGAI241DVIGQTITIS RVEGRRRANE NSNIQVLSER SATEVDNNFS KPPPFFPPGA PPTHLPPPPF301LPPPPTVSTA PPLIPPPGFP PPPGAPPPSL IPTIESGHSS GYDSRSARAF PYGNVAFPHL361PGSAPSWPSL VDTSKQWDYY ARREKDRDRE RDRDRERDRD RDRERERTRE RERERDHSPT421PSVFNSDEER YRYREYAERG YERHRASREK EERHRERRHR EKEETRHKSS RSNSRRRHES481EEGDSHRRHK HKKSKRSKEG KEAGSEPAPE QESTEATPAETRTRPLEQKLISEEDLAANDILDYKDDDDKV
520 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.