Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HFip1 / FIP1L1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125358-100
Catalog Number: LS-G125358
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FIP1L1 (NM_001134938), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Factor interacting with PAP | FIP1-like 1 protein | FIP1 | FIP1 like 1 (S. cerevisiae) | FIP1L1 | HFip1 | RHE
- Origin Species
- Human
- NCBI GenBank
- NM_030917
- SwissProt
- Q6UN15
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSAGEVERLV SELSGGTGGD EEEEWLYGDE NEVERPEEEN ASANPPSGIE DETAENGVPK61PKVTETEDDS DSDSDDDEDD VHVTIGDIKT GAPQYGSYGT APVNLNIKTG GRVYGTTGTK121VKGVDLDAPG SINGVPLLEV DLDSFEDKPW RKPGADLSDY FNYGFNEDTW KAYCEKQKRI181RMGLEVIPVT STTNKITVQQ GRTGNSEKET ALPSTKAEFT SPPSLFKTGL PPSRRLPGAI241DVIGQTITIS RVEGRRRANE NSNIQVLSER SATEVDNNFS KPPPFFPPGA PPTHLPPPPF301LPPPPTVSTA PPLIPPPGFP PPPGAPPPSL IPTIESGHSS GYDSRSARAF PYGNVAFPHL361PGSAPSWPSL VDTSKQWDYY ARREKDRDRE RDRDRERDRD RDRERERTRE RERERDHSPT421PSVFNSDEER YRYREYAERG YERHRASREK EERHRERRHR EKEETRHKSS RSNSRRRHES481EEGDSHRRHK HKKSKRSKEG KEAGSEPAPE QESTEATPAETRTRPLEQKLISEEDLAANDILDYKDDDDKV
520 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.