The activity of recombinant human granzyme A is determined by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB) with an activity of >5,000 pmol/min/µg.

Human GZMA / Granzyme A Protein (Recombinant 10His, C-terminus)

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Expression System	Human Embryonic Kidney 293
Format:	Liquid

$360.00each

Catalog Number: LS-G137846-10

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Overview

Description: GZMA / Granzyme A Protein LS-G137846 is a Recombinant Human GZMA / Granzyme A produced in 293E Cells 26-262 of NP_006135.1 with 10His, C-terminus tag(s). It is low in endotoxin; Less than 1 EU/µg (determined by LAL method). For Research Use Only
Category: Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names: CTLA3 | CTL tryptase | Granzyme-1 | H factor | HF | HFSP | Granzyme A | GZMA | Fragmentin-1 | Hanukah factor | Hanukkah factor
Origin Species: Human
NCBI GenBank: NM_006144
SwissProt: P12544

Protein

Expression System: Human Embryonic Kidney 293
Fusion Tag: Decahistidine Tag
Molecular Weight: This 252 amino acid recombinant protein has a predicted molecular mass of approximately 28 kD. The protein migrates at about 35 kD in DTT-reducing conditions and about 55 kD in non-reducing conditions by SDS-PAGE.The predicted N-terminal amino acid is Cys
Protein Type: Recombinant
Amino Acid Sequence

Format

Purity: >90%
Conjugate/Label: Unconjugated
Endotoxin Level: < 1 EU/µg
Format: Liquid
Formulation: 0.22 µm filtered protein solution in 20 mM Tris, pH 8.0, 150 mM NaCl
Concentration: 0.2 mg/mL
Carrier-Free: Yes
Purity Measurement Method: SDS-PAGE (Coomassie Stain)