Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCNA1 / Cyclin A1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100171-100
Catalog Number: LS-G100171
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cyclin A1(CCNA1) (NM_003914), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCNA1 | Cyclin-A1 | Cyclin A1
- Origin Species
- Human
- NCBI GenBank
- NM_003914
- SwissProt
- P78396
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1METGFPAIMY PGSFIGGWGE EYLSWEGPGL PDFVFQQPVE SEAMHCSNPK SGVVLATVAR61GPDACQILTR APLGQDPPQR TVLGLLTANG QYRRTCGQGI TRIRCYSGSE NAFPPAGKKA121LPDCGVQEPP KQGFDIYMDE LEQGDRDSCS VREGMAFEDV YEVDTGTLKS DLHFLLDFNT181VSPMLVDSSL LSQSEDISSL GTDVINVTEY AEEIYQYLRE AEIRHRPKAH YMKKQPDITE241GMRTILVDWL VEVGEEYKLR AETLYLAVNF LDRFLSCMSV LRGKLQLVGT AAMLLASKYE301EIYPPEVDEF VYITDDTYTK RQLLKMEHLL LKVLAFDLTV PTTNQFLLQY LRRQGVCVRT361ENLAKYVAEL SLLEADPFLK YLPSLIAAAA FCLANYTVNK HFWPETLAAF TGYSLSEIVP421CLSELHKAYL DIPHRPQQAI REKYKASKYL CVSLMEPPAV LLLQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
464 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.