CCNA1 / Cyclin A1 Antibody
Catalog Number: LS-C356843-100
100 µL / $399.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CCNA1 / Cyclin A1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G81730-20
Catalog Number: LS-G81730
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Cyclin A1(CCNA1) (NM_003914) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCNA1 | Cyclin-A1 | Cyclin A1
- Origin Species
- Human
- NCBI GenBank
- NM_003914
- SwissProt
- P78396
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1METGFPAIMY PGSFIGGWGE EYLSWEGPGL PDFVFQQPVE SEAMHCSNPK SGVVLATVAR61GPDACQILTR APLGQDPPQR TVLGLLTANG QYRRTCGQGI TRIRCYSGSE NAFPPAGKKA121LPDCGVQEPP KQGFDIYMDE LEQGDRDSCS VREGMAFEDV YEVDTGTLKS DLHFLLDFNT181VSPMLVDSSL LSQSEDISSL GTDVINVTEY AEEIYQYLRE AEIRHRPKAH YMKKQPDITE241GMRTILVDWL VEVGEEYKLR AETLYLAVNF LDRFLSCMSV LRGKLQLVGT AAMLLASKYE301EIYPPEVDEF VYITDDTYTK RQLLKMEHLL LKVLAFDLTV PTTNQFLLQY LRRQGVCVRT361ENLAKYVAEL SLLEADPFLK YLPSLIAAAA FCLANYTVNK HFWPETLAAF TGYSLSEIVP421CLSELHKAYL DIPHRPQQAI REKYKASKYL CVSLMEPPAV LLLQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
464 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
