Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ACS5 / ACSL5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103100-100
Catalog Number: LS-G103100
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ACSL5 (NM_203379), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACS2 | ACSL5 | ACS5 | FACL5 | Fatty acid coenzyme A ligase 5 | Phosphatidylinositol 4-kinase | LACS 5
- Origin Species
- Human
- NCBI GenBank
- NM_016234
- SwissProt
- Q9ULC5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDALKPPCLW RNHERGKKDR DSCGRKNSEP GSPHSLEALR DAAPSQGLNF LLLFTKMLFI61FNFLFSPLPT PALICILTFG AAIFLWLITR PQPVLPLLDL NNQSVGIEGG ARKGVSQKNN121DLTSCCFSDA KTMYEVFQRG LAVSDNGPCL GYRKPNQPYR WLSYKQVSDR AEYLGSCLLH181KGYKSSPDQF VGIFAQNRPE WIISELACYT YSMVAVPLYD TLGPEAIVHI VNKADIAMVI241CDTPQKALVL IGNVEKGFTP SLKVIILMDP FDDDLKQRGE KSGIEILSLY DAENLGKEHF301RKPVPPSPED LSVICFTSGT TGDPKGAMIT HQNIVSNAAA FLKCVEHAYE PTPDDVAISY361LPLAHMFERI VQAVVYSCGA RVGFFQGDIR LLADDMKTLK PTLFPAVPRL LNRIYDKVQN421EAKTPLKKFL LKLAVSSKFK ELQKGIIRHD SFWDKLIFAK IQDSLGGRVR VIVTGAAPMS481TSVMTFFRAA MGCQVYEAYG QTECTGGCTF TLPGDWTSGH VGVPLACNYV KLEDVADMNY541FTVNNEGEVC IKGTNVFKGY LKDPEKTQEA LDSDGWLHTG DIGRWLPNGT LKIIDRKKNI601FKLAQGEYIA PEKIENIYNR SQPVLQIFVH GESLRSSLVG VVVPDTDVLP SFAAKLGVKG661SFEELCQNQV VREAILEDLQ KIGKESGLKT FEQVKAIFLH PEPFSIENGL LTPTLKAKRG721ELSKYFRTQI DSLYEHIQDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
739 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.