LSBio
ZWILCH Protein (Over-Expression Lysate Myc-DDK (Flag))

ZWILCH Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112083-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZWILCH (NM_017975), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KNTC1AP | Protein zwilch homolog | ZWILCH | Zwilch kinetochore protein | Homolog of Drosophila Zwilch | HZwilch
Origin Species
Human
NCBI GenBank
NM_017975
SwissProt
Q9H900

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWERLNCAAE DFYSRLLQKF NEEKKGIRKD PFLYEADVQV QLISKGQPNP LKNILNENDI
61VFIVEKVPLE KEETSHIEEL QSEETAISDF STGENVGPLA LPVGKARQLI GLYTMAHNPN
121MTHLKINLPV TALPPLWVRC DSSDPEGTCW LGAELITTNN SITGIVLYVV SCKADKNYSV
181NLENLKNLHK KRHHLSTVTS KGFAQYELFK SSALDDTITA SQTAIALDIS WSPVDEILQI
241PPLSSTATLN IKVESGEPRG PLNHLYRELK FLLVLADGLR TGVTEWLEPL EAKSAVELVQ
301EFLNDLNKLD GFGDSTKKDT EVETLKHDTA AVDRSVKRLF KVRSDLDFAE QLWCKMSSSV
361ISYQDLVKCF TLIIQSLQRG DIQPWLHSGS NSLLSKLIHQ SYHGTMDTVS LSGTIPVQML
421LEIGLDKLKK DYISFFIGQE LASLNHLEYF IAPSVDIQEQ VYRVQKLHHI LEILVSCMPF
481IKSQHELLFS LTQICIKYYK QNPLDEQHIF QLPVRPTAVK NLYQSEKPQK WRVEIYSGQK
541KIKTVWQLSD SSPIDHLNFH KPDFSELTLN GSLEERIFFT NMVTCSQVHF K
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

591 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.