ZWILCH Antibody
Catalog Number: LS-C832553
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human, Mouse
Format: Liquid
Human ZWILCH Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G90754-20
Catalog Number: LS-G90754
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ZWILCH (NM_017975) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KNTC1AP | Protein zwilch homolog | ZWILCH | Zwilch kinetochore protein | Homolog of Drosophila Zwilch | HZwilch
- Origin Species
- Human
- NCBI GenBank
- NM_017975
- SwissProt
- Q9H900
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWERLNCAAE DFYSRLLQKF NEEKKGIRKD PFLYEADVQV QLISKGQPNP LKNILNENDI61VFIVEKVPLE KEETSHIEEL QSEETAISDF STGENVGPLA LPVGKARQLI GLYTMAHNPN121MTHLKINLPV TALPPLWVRC DSSDPEGTCW LGAELITTNN SITGIVLYVV SCKADKNYSV181NLENLKNLHK KRHHLSTVTS KGFAQYELFK SSALDDTITA SQTAIALDIS WSPVDEILQI241PPLSSTATLN IKVESGEPRG PLNHLYRELK FLLVLADGLR TGVTEWLEPL EAKSAVELVQ301EFLNDLNKLD GFGDSTKKDT EVETLKHDTA AVDRSVKRLF KVRSDLDFAE QLWCKMSSSV361ISYQDLVKCF TLIIQSLQRG DIQPWLHSGS NSLLSKLIHQ SYHGTMDTVS LSGTIPVQML421LEIGLDKLKK DYISFFIGQE LASLNHLEYF IAPSVDIQEQ VYRVQKLHHI LEILVSCMPF481IKSQHELLFS LTQICIKYYK QNPLDEQHIF QLPVRPTAVK NLYQSEKPQK WRVEIYSGQK541KIKTVWQLSD SSPIDHLNFH KPDFSELTLN GSLEERIFFT NMVTCSQVHF KTRTRPLEQKLISEEDLAANDILDYKDDDDKV
591 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
