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ZNF454 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF454 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G104237-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF454 (NM_182594), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 454 | ZNF454
Origin Species
Human
SwissProt
Q8N9F8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVSHLPTMV QESVTFKDVA ILFTQEEWGQ LSPAQRALYR DVMLENYSNL VSLGLLGPKP
61DTFSQLEKRE VWMPEDTPGG FCLDWMTMPA SKKSTVKAEI PEEELDQWTI KERFSSSSHW
121KCASLLEWQC GGQEISLQRV VLTHPNTPSQ ECDESGSTMS SSLHSAQSQG FQPSKNAFEC
181SECGKVFSKS STLNKHQKIH NEKNANQKIH IKEKRYECRE CGKAFHQSTH LIHHQRIHTG
241EKPYECKECG KAFSVSSSLT YHQKIHTGEK PFECNLCGKA FIRNIHLAHH HRIHTGEKPF
301KCNICEKAFV CRAHLTKHQN IHSGEKPYKC NECGKAFNQS TSFLQHQRIH TGEKPFECNE
361CGKAFRVNSS LTEHQRIHTG EKPYKCNECG KAFRDNSSFA RHRKIHTGEK PYRCGLCEKA
421FRDQSALAQH QRIHTGEKPY TCNICEKAFS DHSALTQHKR IHTREKPYKC KICEKAFIRS
481THLTQHQRIH TGEKPYKCNK CGKAFNQTAN LIQHQRHHIG EK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

522 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.