ZNF454 Antibody (FITC)
Catalog Number: LS-C394806
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ZNF454 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G93779-20
Catalog Number: LS-G93779
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ZNF454 (NM_182594) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Zinc finger protein 454 | ZNF454
- Origin Species
- Human
- SwissProt
- Q8N9F8
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAVSHLPTMV QESVTFKDVA ILFTQEEWGQ LSPAQRALYR DVMLENYSNL VSLGLLGPKP61DTFSQLEKRE VWMPEDTPGG FCLDWMTMPA SKKSTVKAEI PEEELDQWTI KERFSSSSHW121KCASLLEWQC GGQEISLQRV VLTHPNTPSQ ECDESGSTMS SSLHSAQSQG FQPSKNAFEC181SECGKVFSKS STLNKHQKIH NEKNANQKIH IKEKRYECRE CGKAFHQSTH LIHHQRIHTG241EKPYECKECG KAFSVSSSLT YHQKIHTGEK PFECNLCGKA FIRNIHLAHH HRIHTGEKPF301KCNICEKAFV CRAHLTKHQN IHSGEKPYKC NECGKAFNQS TSFLQHQRIH TGEKPFECNE361CGKAFRVNSS LTEHQRIHTG EKPYKCNECG KAFRDNSSFA RHRKIHTGEK PYRCGLCEKA421FRDQSALAQH QRIHTGEKPY TCNICEKAFS DHSALTQHKR IHTREKPYKC KICEKAFIRS481THLTQHQRIH TGEKPYKCNK CGKAFNQTAN LIQHQRHHIG EKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
522 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
