Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF143 / STAF Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117354-100
Catalog Number: LS-G117354
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF143 (NM_003442), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PHZ-1 | SBF | Transcriptional activator Staf | ZNF143 | STAF | Zinc finger protein 143 | HStaf | SPH-binding factor
- Origin Species
- Human
- NCBI GenBank
- NM_003442
- SwissProt
- P52747
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTEFPGGGME AQHVTLCLTE AVTVADGDNL ENMEGVSLQA VTLADGSTAY IQHNSKDAKL61IDGQVIQLED GSAAYVQHVP IPKSTGDSLR LEDGQAVQLE DGTTAFIHHT SKDSYDQSAL121QAVQLEDGTT AYIHHAVQVP QSDTILAIQA DGTVAGLHTG DATIDPDTIS ALEQYAAKVS181IDGSESVAGT GMIGENEQEK KMQIVLQGHA TRVTAKSQQS GEKAFRCEYD GCGKLYTTAH241HLKVHERSHT GDRPYQCEHA GCGKAFATGY GLKSHVRTHT GEKPYRCSED NCTKSFKTSG301DLQKHIRTHT GERPFKCPFE GCGRSFTTSN IRKVHVRTHT GERPYYCTEP GCGRAFASAT361NYKNHVRIHT GEKPYVCTVP GCDKRFTEYS SLYKHHVVHT HSKPYNCNHC GKTYKQISTL421VMHKRTAHND TEPIEEEQEA FFEPPPGQGE DVLKGSQITY VTGVEGDDVV STQVATVTQS481GLSQQVTLIS QDGTQHVNIS QADMQAIGNT ITMVTQDGTP ITVPAHDAVI SSAGTHSVAM541VTAEGTEGQQ VAIVAQDLAA FHTASSEMGH QQHSHHLVTT ETRPLTLVAT SNGTQIAVQL601GEQPSLEEAI RIASRIQQGE TPGLDDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
626 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.