LSBio
Human ZNF143 / STAF Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ZNF143 / STAF Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ZNF143 antibodies →
$153.00each
Catalog Number: LS-G79627-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ZNF143 (NM_003442) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PHZ-1 | SBF | Transcriptional activator Staf | ZNF143 | STAF | Zinc finger protein 143 | HStaf | SPH-binding factor
Origin Species
Human
NCBI GenBank
NM_003442
SwissProt
P52747

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTEFPGGGME AQHVTLCLTE AVTVADGDNL ENMEGVSLQA VTLADGSTAY IQHNSKDAKL
61IDGQVIQLED GSAAYVQHVP IPKSTGDSLR LEDGQAVQLE DGTTAFIHHT SKDSYDQSAL
121QAVQLEDGTT AYIHHAVQVP QSDTILAIQA DGTVAGLHTG DATIDPDTIS ALEQYAAKVS
181IDGSESVAGT GMIGENEQEK KMQIVLQGHA TRVTAKSQQS GEKAFRCEYD GCGKLYTTAH
241HLKVHERSHT GDRPYQCEHA GCGKAFATGY GLKSHVRTHT GEKPYRCSED NCTKSFKTSG
301DLQKHIRTHT GERPFKCPFE GCGRSFTTSN IRKVHVRTHT GERPYYCTEP GCGRAFASAT
361NYKNHVRIHT GEKPYVCTVP GCDKRFTEYS SLYKHHVVHT HSKPYNCNHC GKTYKQISTL
421VMHKRTAHND TEPIEEEQEA FFEPPPGQGE DVLKGSQITY VTGVEGDDVV STQVATVTQS
481GLSQQVTLIS QDGTQHVNIS QADMQAIGNT ITMVTQDGTP ITVPAHDAVI SSAGTHSVAM
541VTAEGTEGQQ VAIVAQDLAA FHTASSEMGH QQHSHHLVTT ETRPLTLVAT SNGTQIAVQL
601GEQPSLEEAI RIASRIQQGE TPGLDD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

626 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.