LSBio
ZIC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZIC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZIC4 antibodies →
$504.00each
Catalog Number: LS-G129708-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZIC4 (NM_001168378), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZIC4 | Zic family member 4 | Zinc finger protein ZIC 4
Origin Species
Human
NCBI GenBank
NM_032153
SwissProt
Q8N9L1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
42.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MPPFCRNAKL GLLSFLICAG RSLLPLGKNA FWLERIENNN TLWEKAQSQK MRYKTSLVMR
61KRLRLYRNTL KESSSSSGHH GPQLTAASSP SVFPGLHEEP PQASPSRPLN GLLRLGLPGD
121MYARPEPFPP GPAARSDALA AAAALHGYGG MNLTVNLAAP HGPGAFFRYM RQPIKQELIC
181KWLAADGTAT PSLCSKTFST MHELVTHVTV EHVGGPEQAN HICFWEECPR QGKPFKAKYK
241LVNHIRVHTG EKPFPCPFPG CGKVFARSEN LKIHKRTHTG EKPFRCEFEG CERRFANSSD
301RKKHSHVHTS DKPYTCKVRG CDKCYTHPSS LRKHMKVHGR SPPPSSGYDS ATPSALVSPS
361SDCGHKSQVA SSAAVAARTA DLSE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

384 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.