ZIC4 Antibody
Catalog Number: LS-C674164
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ZIC4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G92129-20
Catalog Number: LS-G92129
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ZIC4 (NM_001168378) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ZIC4 | Zic family member 4 | Zinc finger protein ZIC 4
- Origin Species
- Human
- NCBI GenBank
- NM_032153
- SwissProt
- Q8N9L1
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPPFCRNAKL GLLSFLICAG RSLLPLGKNA FWLERIENNN TLWEKAQSQK MRYKTSLVMR61KRLRLYRNTL KESSSSSGHH GPQLTAASSP SVFPGLHEEP PQASPSRPLN GLLRLGLPGD121MYARPEPFPP GPAARSDALA AAAALHGYGG MNLTVNLAAP HGPGAFFRYM RQPIKQELIC181KWLAADGTAT PSLCSKTFST MHELVTHVTV EHVGGPEQAN HICFWEECPR QGKPFKAKYK241LVNHIRVHTG EKPFPCPFPG CGKVFARSEN LKIHKRTHTG EKPFRCEFEG CERRFANSSD301RKKHSHVHTS DKPYTCKVRG CDKCYTHPSS LRKHMKVHGR SPPPSSGYDS ATPSALVSPS361SDCGHKSQVA SSAAVAARTA DLSETRTRPLEQKLISEEDLAANDILDYKDDDDKV
384 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
