Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZFAND4 / ANUBL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105034-100
Catalog Number: LS-G105034
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANUBL1(ZFAND4) (NM_174890), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AN1-type zinc finger protein 4 | AN1 | ubiquitin-like | homolog | ANUBL1 | ZFAND4 | Zinc finger | AN1-type domain 4
- Origin Species
- Human
- NCBI GenBank
- AF311324
- SwissProt
- Q86XD8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDNRKEPPFF NDDNMGPFYY RLHFCDTMEL FIETLTGTCF ELRVSPFETV ISVKAKIRRL61EGIPICRQHL IWNNMELEND YCLNDYNISE GCTLKLVLAM RGGPINTRRV PTDDPLRKMA121EYLDSSRVEV WEKTSCSKQV TFLVYQEGDQ LNFFPAVDRG DGTLTPLSDS SKKIDFHLHV181LRRKGEHRMS GGSMYNSDTD EDEETEPSSS GQQIIENSIT MNKMKLLKAK MKNMNLSKKP241KKAVKIKPHP PVAPRPSSGS TAPSRHRLLR VLPNIGQSCS PAFGNAYPPE ISRNGISSLA301TQLSAERYIS SITGEFLKED NSWENNTLSH FSSNVKLPPQ IPHLELGNDQ ELADSVLHLG361SSLPRQTKHF LGNLPSSNGN IVLPSEECVT EQSLLPKVGS LASFAEGNAD EQSSGLEGAC421KVNLELLLTN ADKGLKAPEQ HLKHVAGVLN GESVETSVLN YRELSPHKNR LLSPLRCSAP481MSLHNSLVKP ERQSKCFEFG KLQPSSSQSL DVQNITDSSF SRTTCFQGVK VDSLGKRSDV541ISKVEARDIT EMTNKASKEP VGCVNNISFL ASLAGSTSRN RLQSTRGAGR LQNSGTGLST601NLQHFQEENF RKSSPQLEHT GVFLSTHGVG MNGNNAAAGK SVGECTTHHL PPVKAPLQTK661KKTTNHCFLC GKKTGLASSY ECRCGNNFCA SHRYAETHGC TYDYKSAGRR YLHEANPVVN721APKLPKITRTRPLEQKLISEEDLAANDILDYKDDDDKV
727 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.