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Human ZFAND4 / ANUBL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ZFAND4 / ANUBL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ZFAND4 antibodies →
$153.00each
Catalog Number: LS-G83589-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ANUBL1(ZFAND4) (NM_174890) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AN1-type zinc finger protein 4 | AN1 | ubiquitin-like | homolog | ANUBL1 | ZFAND4 | Zinc finger | AN1-type domain 4
Origin Species
Human
NCBI GenBank
AF311324
SwissProt
Q86XD8

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDNRKEPPFF NDDNMGPFYY RLHFCDTMEL FIETLTGTCF ELRVSPFETV ISVKAKIRRL
61EGIPICRQHL IWNNMELEND YCLNDYNISE GCTLKLVLAM RGGPINTRRV PTDDPLRKMA
121EYLDSSRVEV WEKTSCSKQV TFLVYQEGDQ LNFFPAVDRG DGTLTPLSDS SKKIDFHLHV
181LRRKGEHRMS GGSMYNSDTD EDEETEPSSS GQQIIENSIT MNKMKLLKAK MKNMNLSKKP
241KKAVKIKPHP PVAPRPSSGS TAPSRHRLLR VLPNIGQSCS PAFGNAYPPE ISRNGISSLA
301TQLSAERYIS SITGEFLKED NSWENNTLSH FSSNVKLPPQ IPHLELGNDQ ELADSVLHLG
361SSLPRQTKHF LGNLPSSNGN IVLPSEECVT EQSLLPKVGS LASFAEGNAD EQSSGLEGAC
421KVNLELLLTN ADKGLKAPEQ HLKHVAGVLN GESVETSVLN YRELSPHKNR LLSPLRCSAP
481MSLHNSLVKP ERQSKCFEFG KLQPSSSQSL DVQNITDSSF SRTTCFQGVK VDSLGKRSDV
541ISKVEARDIT EMTNKASKEP VGCVNNISFL ASLAGSTSRN RLQSTRGAGR LQNSGTGLST
601NLQHFQEENF RKSSPQLEHT GVFLSTHGVG MNGNNAAAGK SVGECTTHHL PPVKAPLQTK
661KKTTNHCFLC GKKTGLASSY ECRCGNNFCA SHRYAETHGC TYDYKSAGRR YLHEANPVVN
721APKLPKI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

727 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.