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YTHDF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

YTHDF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112213-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human YTHDF1 (NM_017798), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C20orf21 | DACA-1 | YTHDF1 | YTH domain family | member 1 | YTH domain family 1 | YTH domain family protein 1
Origin Species
Human
NCBI GenBank
NM_017798
SwissProt
Q9BYJ9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSATSVDTQR TKGQDNKVQN GSLHQKDTVH DNDFEPYLTG QSNQSNSYPS MSDPYLSSYY
61PPSIGFPYSL NEAPWSTAGD PPIPYLTTYG QLSNGDHHFM HDAVFGQPGG LGNNIYQHRF
121NFFPENPAFS AWGTSGSQGQ QTQSSAYGSS YTYPPSSLGG TVVDGQPGFH SDTLSKAPGM
181NSLEQGMVGL KIGDVSSSAV KTVGSVVSSV ALTGVLSGNG GTNVNMPVSK PTSWAAIASK
241PAKPQPKMKT KSGPVMGGGL PPPPIKHNMD IGTWDNKGPV PKAPVPQQAP SPQAAPQPQQ
301VAQPLPAQPP ALAQPQYQSP QQPPQTRWVA PRNRNAAFGQ SGGAGSDSNS PGNVQPNSAP
361SVESHPVLEK LKAAHSYNPK EFEWNLKSGR VFIIKSYSED DIHRSIKYSI WCSTEHGNKR
421LDSAFRCMSS KGPVYLLFSV NGSGHFCGVA EMKSPVDYGT SAGVWSQDKW KGKFDVQWIF
481VKDVPNNQLR HIRLENNDNK PVTNSRDTQE VPLEKAKQVL KIISSYKHTT SIFDDFAHYE
541KRQEEEEVVR KERQSRNKQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

559 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.