Western blot analysis of extracts of various cell lines, using YTHDF1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

YTHDF1 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunofluorescence, Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

$281.00each

Catalog Number: LS-C748320-20

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Overview

Description: YTHDF1 antibody LS-C748320 is an unconjugated rabbit polyclonal antibody to YTHDF1 from human. It is reactive with human, mouse and rat. Validated for IF and WB.
Application: IF, WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: C20orf21 | DACA-1 | YTHDF1 | YTH domain family, member 1 | YTH domain family 1 | YTH domain family protein 1
Recommended Dilution: IF/ICC: 1:50 - 1:200
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q9BYJ9
NCBI GenBank: NM_017798 | NP_060268.2
SwissProt: Q9BYJ9

Target

Target: Human YTHDF1
Antigen Species: Human
Epitope: Human YTHDF1
Gene Name: YTHDF1

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human YTHDF1 (NP_060268.2). MSATSVDTQRTKGQDNKVQNGSLHQKDTVHDNDFEPYLTGQSNQSNSYPSMSDPYLSSYYPPSIGFPYSLNEAPWSTAGDPPIPYLTTYGQLSNGDHHFMHDAVFGQPGGLGNNIYQHRFNFFPENPAFSAWGTSGSQGQQTQSSAYGSSYTYPPSSLGGTVVDGQPGFHSDTLSKAPGMNSLEQGMVGLKIGDVSSSAV
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)