Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
WT1 / Wilms Tumor 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109977-100
Catalog Number: LS-G109977
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Wilms Tumor Protein(WT1) (NM_024425), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GUD | EWS-WT1 | Amino-terminal domain of EWS | WAGR | WIT-2 | Wilms tumor protein | WT1 | AWT1 | Wilms tumor 1 | WT33 | NPHS4 | WT-1
- Origin Species
- Human
- NCBI GenBank
- NM_024426
- SwissProt
- P19544
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1LQDPASTCVP EPASQHTLRS GPGCLQQPEQ QGVRDPGGIW AKLGAAEASA ERLQGRRSRG61ASGSEPQQMG SDVRDLNALL PAVPSLGGGG GCALPVSGAA QWAPVLDFAP PGASAYGSLG121GPAPPPAPPP PPPPPPHSFI KQEPSWGGAE PHEEQCLSAF TVHFSGQFTG TAGACRYGPF181GPPPPSQASS GQARMFPNAP YLPSCLESQP AIRNQGYSTV TFDGTPSYGH TPSHHAAQFP241NHSFKHEDPM GQQGSLGEQQ YSVPPPVYGC HTPTDSCTGS QALLLRTPYS SDNLYQMTSQ301LECMTWNQMN LGATLKGHST GYESDNHTTP ILCGAQYRIH THGVFRGIQD VRRVPGVAPT361LVRSASETSE KRPFMCAYPG CNKRYFKLSH LQMHSRKHTG EKPYQCDFKD CERRFSRSDQ421LKRHQRRHTG VKPFQCKTCQ RKFSRSDHLK THTRTHTGKT SEKPFSCRWP SCQKKFARSD481ELVRHHNMHQ RNMTKLQLALTRTRPLEQKLISEEDLAANDILDYKDDDDKV
500 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.