WT1 / Wilms Tumor 1 Antibody (clone WT1/857)
Catalog Number: LS-C391137
2 sizes / from $379.00
Antibody: Mouse Monoclonal
Applications: Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human WT1 / Wilms Tumor 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G87357-20
Catalog Number: LS-G87357
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Wilms Tumor Protein(WT1) (NM_024425) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GUD | EWS-WT1 | Amino-terminal domain of EWS | WAGR | WIT-2 | Wilms tumor protein | WT1 | AWT1 | Wilms tumor 1 | WT33 | NPHS4 | WT-1
- Origin Species
- Human
- NCBI GenBank
- NM_024426
- SwissProt
- P19544
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1LQDPASTCVP EPASQHTLRS GPGCLQQPEQ QGVRDPGGIW AKLGAAEASA ERLQGRRSRG61ASGSEPQQMG SDVRDLNALL PAVPSLGGGG GCALPVSGAA QWAPVLDFAP PGASAYGSLG121GPAPPPAPPP PPPPPPHSFI KQEPSWGGAE PHEEQCLSAF TVHFSGQFTG TAGACRYGPF181GPPPPSQASS GQARMFPNAP YLPSCLESQP AIRNQGYSTV TFDGTPSYGH TPSHHAAQFP241NHSFKHEDPM GQQGSLGEQQ YSVPPPVYGC HTPTDSCTGS QALLLRTPYS SDNLYQMTSQ301LECMTWNQMN LGATLKGHST GYESDNHTTP ILCGAQYRIH THGVFRGIQD VRRVPGVAPT361LVRSASETSE KRPFMCAYPG CNKRYFKLSH LQMHSRKHTG EKPYQCDFKD CERRFSRSDQ421LKRHQRRHTG VKPFQCKTCQ RKFSRSDHLK THTRTHTGKT SEKPFSCRWP SCQKKFARSD481ELVRHHNMHQ RNMTKLQLALTRTRPLEQKLISEEDLAANDILDYKDDDDKV
500 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
