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WDR47 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR47 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR47 antibodies →
$725.00each
Catalog Number: LS-G126005-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR47 (NM_001142550), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0893 | WDR47 | Nemitin | WD repeat domain 47
Origin Species
Human
NCBI GenBank
NM_014969
SwissProt
O94967

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
102.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTAEETVNVK EVEIIKLILD FLNSKKLHIS MLALEKESGV INGLFSDDML FLRQLILDGQ
61WDEVLQFIQP LECMEKFDKK RFRYIILKQK FLEALCVNNA MSAEDEPQHV RFLFLKLEFT
121MQEAVQCLHA LEEYCPSKDD YSKLCLLLTL PRLTNHAEFK DWNPSTARVH CFEEACVMVA
181EFIPADRKLS EAGFKASNNR LFQLVMKGLL YECCVEFCQS KATGEEITES EVLLGIDLLC
241GNGCDDLDLS LLSWLQNLPS SVFSCAFEQK MLNIHVDKLL KPTKAAYADL LTPLISKLSP
301YPSSPMRRPQ SADAYMTRSL NPALDGLTCG LTSHDKRISD LGNKTSPMSH SFANFHYPGV
361QNLSRSLMLE NTECHSIYEE SPERSDTPVD AQRPIGSEIL GQSSVSEKEP ANGAQNPGPA
421KQEKNELRDS TEQFQEYYRQ RLRYQQHLEQ KEQQRQIYQQ MLLEGGVNQE DGPDQQQNLT
481EQFLNRSIQK LGELNIGMDG LGNEVSALNQ QCNGSKGNGS NGSSVTSFTT PPQDSSQRLT
541HDASNIHTST PRNPGSTNHI PFLEESPCGS QISSEHSVIK PPLGDSPGSL SRSKGEEDDK
601SKKQFVCINI LEDTQAVRAV AFHPAGGLYA VGSNSKTLRV CAYPDVIDPS AHETPKQPVV
661RFKRNKHHKG SIYCVAWSPC GQLLATGSND KYVKVLPFNA ETCNATGPDL EFSMHDGTIR
721DLAFMEGPES GGAILISAGA GDCNIYTTDC QRGQGLHALS GHTGHILALY TWSGWMIASG
781SQDKTVRFWD LRVPSCVRVV GTTFHGTGSA VASVAVDPSG RLLATGQEDS SCMLYDIRGG
841RMVQSYHPHS SDVRSVRFSP GAHYLLTGSY DMKIKVTDLQ GDLTKQLPIM VVGEHKDKVI
901QCRWHTQDLS FLSSSADRTV TLWTYNG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

927 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.