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WDR47 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR47 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR47 antibodies →
$504.00each
Catalog Number: LS-G113535-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR47 (NM_014969), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0893 | WDR47 | Nemitin | WD repeat domain 47
Origin Species
Human
NCBI GenBank
NM_014969
SwissProt
O94967

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
101.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTAEETVNVK EVEIIKLILD FLNSKKLHIS MLALEKESGV INGLFSDDML FLRQLILDGQ
61WDEVLQFIQP LECMEKFDKK RFRYIILKQK FLEALCVNNA MSAEDEPQHL EFTMQEAVQC
121LHALEEYCPS KDDYSKLCLL LTLPRLTNHA EFKDWNPSTA RVHCFEEACV MVAEFIPADR
181KLSEAGFKAS NNRLFQLVMK GLLYECCVEF CQSKATGEEI TESEVLLGID LLCGNGCDDL
241DLSLLSWLQN LPSSVFSCAF EQKMLNIHVD KLLKPTKAAY ADLLTPLISK LSPYPSSPMR
301RPQSADAYMT RSLNPALDGL TCGLTSHDKR ISDLGNKTSP MSHSFANFHY PGVQNLSRSL
361MLENTECHSI YEESPERSDT PVDAQRPIGS EILGQSSVSE KEPANGAQNP GPAKQEKNEL
421RDSTEQFQEY YRQRLRYQQH LEQKEQQRQI YQQMLLEGGV NQEDGPDQQQ NLTEQFLNRS
481IQKLGELNIG MDGLGNEVSA LNQQCNGSKG NGSNGSSVTS FTTPPQDSSQ RLTHDASNIH
541TSTPRNPGST NHIPFLEESP CGSQISSEHS VIKPPLGDSP GSLSRSKGEE DDKSKKQFVC
601INILEDTQAV RAVAFHPAGG LYAVGSNSKT LRVCAYPDVI DPSAHETPKQ PVVRFKRNKH
661HKGSIYCVAW SPCGQLLATG SNDKYVKVLP FNAETCNATG PDLEFSMHDG TIRDLAFMEG
721PESGGAILIS AGAGDCNIYT TDCQRGQGLH ALSGHTGHIL ALYTWSGWMI ASGSQDKTVR
781FWDLRVPSCV RVVGTTFHGT GSAVASVAVD PSGRLLATGQ EDSSCMLYDI RGGRMVQSYH
841PHSSDVRSVR FSPGAHYLLT GSYDMKIKVT DLQGDLTKQL PIMVVGEHKD KVIQCRWHTQ
901DLSFLSSSAD RTVTLWTYNG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

920 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.