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WDR35 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR35 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR35 antibodies →
$504.00each
Catalog Number: LS-G111018-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR35 (NM_020779), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CED2 | IFT121 | KIAA1336 | WD repeat domain 35 | WDR35 | Naofen
Origin Species
Human
NCBI GenBank
AB037757
SwissProt
Q9P2L0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
132.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFFYLSKKIS IPNNVKLQCV SWNKEQGFIA CGGEDGLLKV LKLETQTDDA KLRGLAAPSN
61LSMNQTLEGH SGSVQVVTWN EQYQKLTTSD ENGLIIVWML YKGSWIEEMI NNRNKSVVRS
121MSWNADGQKI CIVYEDGAVI VGSVDGNRIW GKDLKGIQLS HVTWSADSKV LLFGMANGEI
181HIYDNQGNFM IKMKLSCLVN VTGAISIAGI HWYHGTEGYV EPDCPCLAVC FDNGRCQIMR
241HENDQNPVLI DTGMYVVGIQ WNHMGSVLAV AGFQKAAMQD KDVNIVQFYT PFGEHLGTLK
301VPGKEISALS WEGGGLKIAL AVDSFIYFAN IRPNYKWGYC SNTVVYAYTR PDRPEYCVVF
361WDTKNNEKYV KYVKGLISIT TCGDFCILAT KADENHPQFV LVLCNSIGTP LDPKYIDIVP
421LFVAMTKTHV IAASKEAFYT WQYRVAKKLT ALEINQITRS RKEGRERIYH VDDTPSGSMD
481GVLDYSKTIQ GTRDPICAIT ASDKILIVGR ESGTIQRYSL PNVGLIQKYS LNCRAYQLSL
541NCNSSRLAII DISGVLTFFD LDARVTDSTG QQVVGELLKL ERRDVWDMKW AKDNPDLFAM
601MEKTRMYVFR NLDPEEPIQT SGYICNFEDL EIKSVLLDEI LKDPEHPNKD YLINFEIRSL
661RDSRALIEKV GIKDASQFIE DNPHPRLWRL LAEAALQKLD LYTAEQAFVR CKDYQGIKFV
721KRLGKLLSES MKQAEVVGYF GRFEEAERTY LEMDRRDLAI GLRLKLGDWF RVLQLLKTGS
781GDADDSLLEQ ANNAIGDYFA DRQKWLNAVQ YYVQGRNQER LAECYYMLED YEGLENLAIS
841LPENHKLLPE IAQMFVRVGM CEQAVTAFLK CSQPKAAVDT CVHLNQWNKA VELAKNHSMK
901EIGSLLARYA SHLLEKNKTL DAIELYRKAN YFFDAAKLMF KIADEEAKKG SKPLRVKKLY
961VLSALLIEQY HEQMKNAQRG KVKGKSSEAT SALAGLLEEE VLSTTDRFTD NAWRGAEAYH
1021FFILAQRQLY EGCVDTALKT ALHLKDYEDI IPPVEIYSLL ALCACASRAF GTCSKAFIKL
1081KSLETLSSEQ KQQYEDLALE IFTKHTSKDN RKPELDSLME GGEGKLPTCV ATGSPITEYQ
1141FWMCSVCKHG VLAQEISHYS FCPLCHSPVG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1170 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.