WDR35 Antibody
Catalog Number: LS-C747895
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human WDR35 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G82511-20
Catalog Number: LS-G82511
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human WDR35 (NM_020779) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CED2 | IFT121 | KIAA1336 | WD repeat domain 35 | WDR35 | Naofen
- Origin Species
- Human
- NCBI GenBank
- AB037757
- SwissProt
- Q9P2L0
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 132.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFFYLSKKIS IPNNVKLQCV SWNKEQGFIA CGGEDGLLKV LKLETQTDDA KLRGLAAPSN61LSMNQTLEGH SGSVQVVTWN EQYQKLTTSD ENGLIIVWML YKGSWIEEMI NNRNKSVVRS121MSWNADGQKI CIVYEDGAVI VGSVDGNRIW GKDLKGIQLS HVTWSADSKV LLFGMANGEI181HIYDNQGNFM IKMKLSCLVN VTGAISIAGI HWYHGTEGYV EPDCPCLAVC FDNGRCQIMR241HENDQNPVLI DTGMYVVGIQ WNHMGSVLAV AGFQKAAMQD KDVNIVQFYT PFGEHLGTLK301VPGKEISALS WEGGGLKIAL AVDSFIYFAN IRPNYKWGYC SNTVVYAYTR PDRPEYCVVF361WDTKNNEKYV KYVKGLISIT TCGDFCILAT KADENHPQFV LVLCNSIGTP LDPKYIDIVP421LFVAMTKTHV IAASKEAFYT WQYRVAKKLT ALEINQITRS RKEGRERIYH VDDTPSGSMD481GVLDYSKTIQ GTRDPICAIT ASDKILIVGR ESGTIQRYSL PNVGLIQKYS LNCRAYQLSL541NCNSSRLAII DISGVLTFFD LDARVTDSTG QQVVGELLKL ERRDVWDMKW AKDNPDLFAM601MEKTRMYVFR NLDPEEPIQT SGYICNFEDL EIKSVLLDEI LKDPEHPNKD YLINFEIRSL661RDSRALIEKV GIKDASQFIE DNPHPRLWRL LAEAALQKLD LYTAEQAFVR CKDYQGIKFV721KRLGKLLSES MKQAEVVGYF GRFEEAERTY LEMDRRDLAI GLRLKLGDWF RVLQLLKTGS781GDADDSLLEQ ANNAIGDYFA DRQKWLNAVQ YYVQGRNQER LAECYYMLED YEGLENLAIS841LPENHKLLPE IAQMFVRVGM CEQAVTAFLK CSQPKAAVDT CVHLNQWNKA VELAKNHSMK901EIGSLLARYA SHLLEKNKTL DAIELYRKAN YFFDAAKLMF KIADEEAKKG SKPLRVKKLY961VLSALLIEQY HEQMKNAQRG KVKGKSSEAT SALAGLLEEE VLSTTDRFTD NAWRGAEAYH1021FFILAQRQLY EGCVDTALKT ALHLKDYEDI IPPVEIYSLL ALCACASRAF GTCSKAFIKL1081KSLETLSSEQ KQQYEDLALE IFTKHTSKDN RKPELDSLME GGEGKLPTCV ATGSPITEYQ1141FWMCSVCKHG VLAQEISHYS FCPLCHSPVGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1170 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
