Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
WDR21A / DCAF4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113121-100
Catalog Number: LS-G113121
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR21A(DCAF4) (NM_015604), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DCAF4 | WD repeat domain 21 | WDR21 | WDR21A | WD repeat domain 21A
- Origin Species
- Human
- NCBI GenBank
- N52666
- SwissProt
- Q8WV16
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNKSRWQSRR RHGRRSHQQN PWFRLRDSED RSDSRAAQPA HDSGHGDDES PSTSSGTAGT61SSVPELPGFY FDPEKKRYFR LLPGHNNCNP LTKESIRQKE MESKRLRLLQ EEDRRKKIAR121MGFNASSMLR KSQLGFLNVT NYCHLAHELR LSCMERKKVQ IRSMDPSALA SDRFNLILAD181TNSDRLFTVN DVTVGGSKYG IINLQSLKTP TLKVFMHENL YFTNRKVNSV CWASLNHLDS241HILLCLMGLA ETPGCATLLP ASLFVNSHPG IDRPGMLCSF RIPGAWSCAW SLNIQANNCF301STGLSRRVLL TNVVTGHRQS FGTNSDVLAQ QFAFMAPLLF NGCRSGEIFA IDLRCGNQGK361GWKATRLFHD SAVTSVRILQ DEQYLMASDM AGKIKLWDLR TTKCVRQYEG HVNEYAYLPL421HVHEEEGILV AVGQDCYTII WSLHDARLLR TIPSPYPASK ADIPSVAFSS RLGGSRGAPG481LLMAVGQDLY CYSYSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
495 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.