WDR21A / DCAF4 Antibody
Catalog Number: LS-C749851
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human WDR21A / DCAF4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G86367-20
Catalog Number: LS-G86367
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human WDR21A(DCAF4) (NM_015604) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DCAF4 | WD repeat domain 21 | WDR21 | WDR21A | WD repeat domain 21A
- Origin Species
- Human
- NCBI GenBank
- N52666
- SwissProt
- Q8WV16
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNKSRWQSRR RHGRRSHQQN PWFRLRDSED RSDSRAAQPA HDSGHGDDES PSTSSGTAGT61SSVPELPGFY FDPEKKRYFR LLPGHNNCNP LTKESIRQKE MESKRLRLLQ EEDRRKKIAR121MGFNASSMLR KSQLGFLNVT NYCHLAHELR LSCMERKKVQ IRSMDPSALA SDRFNLILAD181TNSDRLFTVN DVTVGGSKYG IINLQSLKTP TLKVFMHENL YFTNRKVNSV CWASLNHLDS241HILLCLMGLA ETPGCATLLP ASLFVNSHPG IDRPGMLCSF RIPGAWSCAW SLNIQANNCF301STGLSRRVLL TNVVTGHRQS FGTNSDVLAQ QFAFMAPLLF NGCRSGEIFA IDLRCGNQGK361GWKATRLFHD SAVTSVRILQ DEQYLMASDM AGKIKLWDLR TTKCVRQYEG HVNEYAYLPL421HVHEEEGILV AVGQDCYTII WSLHDARLLR TIPSPYPASK ADIPSVAFSS RLGGSRGAPG481LLMAVGQDLY CYSYSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
495 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
