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VIT Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VIT Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G130153-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VIT (NM_001177969), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_053276

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRTVVLTMKA SVIEMFLVLL VTGVHSNKET AKKIKRPKFT VPQINCDVKA GKIIDPEFIV
61KCPAGCQDPK YHVYGTDVYA SYSSVCGAAV HSGVLDNSGG KILVRKVAGQ SGYKGSYSNG
121VQSLSLPRWR ESFIVLESKP KKGVTYPSAL TYSSSKSPAA QAGETTKAYQ RPPIPGTTAQ
181PVTLMQLLAV TVAVATPTTL PRPSPSAAST TSIPRPQSVG HRSQEMDLWS TATYTSSQNR
241PRADPGIQRQ DPSGAAFQKP VGADVSLGLV PKEELSTQSL EPVSLGDPNC KIDLSFLIDG
301STSIGKRRFR IQKQLLADVA QALDIGPAGP LMGVVQYGDN PATHFNLKTH TNSRDLKTAI
361EKITQRGGLS NVGRAISFVT KNFFSKANGN RSGAPNVVVV MVDGWPTDKV EEASRLARES
421GINIFFITIE GAAENEKQYV VEPNFANKAV CRTNGFYSLH VQSWFGLHKT LQPLVKRVCD
481TDRLACSKTC LNSADIGFVI DGSSSVGTGN FRTVLQFVTN LTKEFEISDT DTRIGAVQYT
541YEQRLEFGFD KYSSKPDILN AIKRVGYWSG GTSTGAAINF ALEQLFKKSK PNKRKLMILI
601TDGRSYDDVR IPAMAAHLKG VITYAIGVAW AAQEELEVIA THPARDHSFF VDEFDNLHQY
661VPRIIQNICT EFNSQPRN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

678 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.