VIT Antibody (aa567-595)
Catalog Number: LS-C344101-400
400 µL / $401.00
Antibody: Rabbit Polyclonal
Applications: Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human VIT Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G96278-20
Catalog Number: LS-G96278
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human VIT (NM_001177969) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_053276
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRTVVLTMKA SVIEMFLVLL VTGVHSNKET AKKIKRPKFT VPQINCDVKA GKIIDPEFIV61KCPAGCQDPK YHVYGTDVYA SYSSVCGAAV HSGVLDNSGG KILVRKVAGQ SGYKGSYSNG121VQSLSLPRWR ESFIVLESKP KKGVTYPSAL TYSSSKSPAA QAGETTKAYQ RPPIPGTTAQ181PVTLMQLLAV TVAVATPTTL PRPSPSAAST TSIPRPQSVG HRSQEMDLWS TATYTSSQNR241PRADPGIQRQ DPSGAAFQKP VGADVSLGLV PKEELSTQSL EPVSLGDPNC KIDLSFLIDG301STSIGKRRFR IQKQLLADVA QALDIGPAGP LMGVVQYGDN PATHFNLKTH TNSRDLKTAI361EKITQRGGLS NVGRAISFVT KNFFSKANGN RSGAPNVVVV MVDGWPTDKV EEASRLARES421GINIFFITIE GAAENEKQYV VEPNFANKAV CRTNGFYSLH VQSWFGLHKT LQPLVKRVCD481TDRLACSKTC LNSADIGFVI DGSSSVGTGN FRTVLQFVTN LTKEFEISDT DTRIGAVQYT541YEQRLEFGFD KYSSKPDILN AIKRVGYWSG GTSTGAAINF ALEQLFKKSK PNKRKLMILI601TDGRSYDDVR IPAMAAHLKG VITYAIGVAW AAQEELEVIA THPARDHSFF VDEFDNLHQY661VPRIIQNICT EFNSQPRNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
678 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
