Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
USP33 / VDU1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103258-100
Catalog Number: LS-G103258
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human USP33 (NM_201624), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Deubiquitinating enzyme 33 | KIAA1097 | HVDU1 | USP33 | VDU1 | Ubiquitin thioesterase 33 | Ubiquitin thiolesterase 33 | Ubiquitin specific protease 33
- Origin Species
- Human
- NCBI GenBank
- NM_015017
- SwissProt
- Q8TEY7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 103.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSAFRNHCPH LDSVGEITKE DLIQKSLGTC QDCKVQGPNL WACLENRCSY VGCGESQVDH61STIHSQETKH YLTVNLTTLR VWCYACSKEV FLDRKLGTQP SLPHVRQPHQ IQENSVQDFK121IPSNTTLKTP LVAVFDDLDI EADEEDELRA RGLTGLKNIG NTCYMNAALQ ALSNCPPLTQ181FFLDCGGLAR TDKKPAICKS YLKLMTELWH KSRPGSVVPT TLFQGIKTVN PTFRGYSQQD241AQEFLRCLMD LLHEELKEQV MEVEEDPQTI TTEETMEEDK SQSDVDFQSC ESCSNSDRAE301NENGSRCFSE DNNETTMLIQ DDENNSEMSK DWQKEKMCNK INKVNSEGEF DKDRDSISET361VDLNNQETVK VQIHSRASEY ITDVHSNDLS TPQILPSNEG VNPRLSASPP KSGNLWPGLA421PPHKKAQSAS PKRKKQHKKY RSVISDIFDG TIISSVQCLT CDRVSVTLET FQDLSLPIPG481KEDLAKLHSS SHPTSIVKAG SCGEAYAPQG WIAFFMEYVK RFVVSCVPSW FWGPVVTLQD541CLAAFFARDE LKGDNMYSCE KCKKLRNGVK FCKVQNFPEI LCIHLKRFRH ELMFSTKIST601HVSFPLEGLD LQPFLAKDSP AQIVTYDLLS VICHHGTASS GHYIAYCRNN LNNLWYEFDD661QSVTEVSEST VQNAEAYVLF YRKSSEEAQK ERRRISNLLN IMEPSLLQFY ISRQWLNKFK721TFAEPGPISN NDFLCIHGGV PPRKAGYIED LVLMLPQNIW DNLYSRYGGG PAVNHLYICH781TCQIEAEKIE KRRKTELEIF IRLNRAFQKE DSPATFYCIS MQWFREWESF VKGKDGDPPG841PIDNTKIAVT KCGNVMLRQG ADSGQISEET WNFLQSIYGG GPEVILRPPV VHVDPDILQA901EEKIEVETRS LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
911 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.