LSBio
Human USP33 / VDU1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human USP33 / VDU1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all USP33 antibodies →
$220.00each
Catalog Number: LS-G82386-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human USP33 (NM_201624) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Deubiquitinating enzyme 33 | KIAA1097 | HVDU1 | USP33 | VDU1 | Ubiquitin thioesterase 33 | Ubiquitin thiolesterase 33 | Ubiquitin specific protease 33
Origin Species
Human
NCBI GenBank
NM_015017
SwissProt
Q8TEY7

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
103.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSAFRNHCPH LDSVGEITKE DLIQKSLGTC QDCKVQGPNL WACLENRCSY VGCGESQVDH
61STIHSQETKH YLTVNLTTLR VWCYACSKEV FLDRKLGTQP SLPHVRQPHQ IQENSVQDFK
121IPSNTTLKTP LVAVFDDLDI EADEEDELRA RGLTGLKNIG NTCYMNAALQ ALSNCPPLTQ
181FFLDCGGLAR TDKKPAICKS YLKLMTELWH KSRPGSVVPT TLFQGIKTVN PTFRGYSQQD
241AQEFLRCLMD LLHEELKEQV MEVEEDPQTI TTEETMEEDK SQSDVDFQSC ESCSNSDRAE
301NENGSRCFSE DNNETTMLIQ DDENNSEMSK DWQKEKMCNK INKVNSEGEF DKDRDSISET
361VDLNNQETVK VQIHSRASEY ITDVHSNDLS TPQILPSNEG VNPRLSASPP KSGNLWPGLA
421PPHKKAQSAS PKRKKQHKKY RSVISDIFDG TIISSVQCLT CDRVSVTLET FQDLSLPIPG
481KEDLAKLHSS SHPTSIVKAG SCGEAYAPQG WIAFFMEYVK RFVVSCVPSW FWGPVVTLQD
541CLAAFFARDE LKGDNMYSCE KCKKLRNGVK FCKVQNFPEI LCIHLKRFRH ELMFSTKIST
601HVSFPLEGLD LQPFLAKDSP AQIVTYDLLS VICHHGTASS GHYIAYCRNN LNNLWYEFDD
661QSVTEVSEST VQNAEAYVLF YRKSSEEAQK ERRRISNLLN IMEPSLLQFY ISRQWLNKFK
721TFAEPGPISN NDFLCIHGGV PPRKAGYIED LVLMLPQNIW DNLYSRYGGG PAVNHLYICH
781TCQIEAEKIE KRRKTELEIF IRLNRAFQKE DSPATFYCIS MQWFREWESF VKGKDGDPPG
841PIDNTKIAVT KCGNVMLRQG ADSGQISEET WNFLQSIYGG GPEVILRPPV VHVDPDILQA
901EEKIEVETRS L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

911 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.