Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
USP20 / VDU2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121840-100
Catalog Number: LS-G121840
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human USP20 (NM_001008563), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Deubiquitinating enzyme 20 | Ubiquitin specific protease 20 | Ubiquitin thioesterase 20 | USP20 | HVDU2 | KIAA1003 | LSFR3A | Ubiquitin thiolesterase 20 | VDU2
- Origin Species
- Human
- NCBI GenBank
- NM_006676
- SwissProt
- Q9Y2K6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 101.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGDSRDLCPH LDSIGEVTKE DLLLKSKGTC QSCGVTGPNL WACLQVACPY VGCGESFADH61STIHAQAKKH NLTVNLTTFR LWCYACEKEV FLEQRLAAPL LGSSSKFSEQ DSPPPSHPLK121AVPIAVADEG ESESEDDDLK PRGLTGMKNL GNSCYMNAAL QALSNCPPLT QFFLECGGLV181RTDKKPALCK SYQKLVSEVW HKKRPSYVVP TSLSHGIKLV NPMFRGYAQQ DTQEFLRCLM241DQLHEELKEP VVATVALTEA RDSDSSDTDE KREGDRSPSE DEFLSCDSSS DRGEGDGQGR301GGGSSQAETE LLIPDEAGRA ISEKERMKDR KFSWGQQRTN SEQVDEDADV DTAMAALDQP361AEAQPPSPRS SSPCRTPEPD NDAHLRSSSR PCSPVHHHEG HAKLSSSPPR ASPVRMAPSY421VLKKAQVLSA GSRRRKEQRY RSVISDIFDG SILSLVQCLT CDRVSTTVET FQDLSLPIPG481KEDLAKLHSA IYQNVPAKPG ACGDSYAAQG WLAFIVEYIR RFVVSCTPSW FWGPVVTLED541CLAAFFAADE LKGDNMYSCE RCKKLRNGVK YCKVLRLPEI LCIHLKRFRH EVMYSFKINS601HVSFPLEGLD LRPFLAKECT SQITTYDLLS VICHHGTAGS GHYIAYCQNV INGQWYEFDD661QYVTEVHETV VQNAEGYVLF YRKSSEEAMR ERQQVVSLAA MREPSLLRFY VSREWLNKFN721TFAEPGPITN QTFLCSHGGI PPHKYHYIDD LVVILPQNVW EHLYNRFGGG PAVNHLYVCS781ICQVEIEALA KRRRIEIDTF IKLNKAFQAE ESPGVIYCIS MQWFREWEAF VKGKDNEPPG841PIDNSRIAQV KGSGHVQLKQ GADYGQISEE TWTYLNSLYG GGPEIAIRQS VAQPLGPENL901HGEQKIEAET RAVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
913 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.