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Human USP20 / VDU2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human USP20 / VDU2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all USP20 antibodies →
$153.00each
Catalog Number: LS-G87050-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human USP20 (NM_006676) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Deubiquitinating enzyme 20 | Ubiquitin specific protease 20 | Ubiquitin thioesterase 20 | USP20 | HVDU2 | KIAA1003 | LSFR3A | Ubiquitin thiolesterase 20 | VDU2
Origin Species
Human
NCBI GenBank
NM_006676
SwissProt
Q9Y2K6

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
101.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGDSRDLCPH LDSIGEVTKE DLLLKSKGTC QSCGVTGPNL WACLQVACPY VGCGESFADH
61STIHAQAKKH NLTVNLTTFR LWCYACEKEV FLEQRLAAPL LGSSSKFSEQ DSPPPSHPLK
121AVPIAVADEG ESESEDDDLK PRGLTGMKNL GNSCYMNAAL QALSNCPPLT QFFLECGGLV
181RTDKKPALCK SYQKLVSEVW HKKRPSYVVP TSLSHGIKLV NPMFRGYAQQ DTQEFLRCLM
241DQLHEELKEP VVATVALTEA RDSDSSDTDE KREGDRSPSE DEFLSCDSSS DRGEGDGQGR
301GGGSSQAETE LLIPDEAGRA ISEKERMKDR KFSWGQQRTN SEQVDEDADV DTAMAALDQP
361AEAQPPSPRS SSPCRTPEPD NDAHLRSSSR PCSPVHHHEG HAKLSSSPPR ASPVRMAPSY
421VLKKAQVLSA GSRRRKEQRY RSVISDIFDG SILSLVQCLT CDRVSTTVET FQDLSLPIPG
481KEDLAKLHSA IYQNVPAKPG ACGDSYAAQG WLAFIVEYIR RFVVSCTPSW FWGPVVTLED
541CLAAFFAADE LKGDNMYSCE RCKKLRNGVK YCKVLRLPEI LCIHLKRFRH EVMYSFKINS
601HVSFPLEGLD LRPFLAKECT SQITTYDLLS VICHHGTAGS GHYIAYCQNV INGQWYEFDD
661QYVTEVHETV VQNAEGYVLF YRKSSEEAMR ERQQVVSLAA MREPSLLRFY VSREWLNKFN
721TFAEPGPITN QTFLCSHGGI PPHKYHYIDD LVVILPQNVW EHLYNRFGGG PAVNHLYVCS
781ICQVEIEALA KRRRIEIDTF IKLNKAFQAE ESPGVIYCIS MQWFREWEAF VKGKDNEPPG
841PIDNSRIAQV KGSGHVQLKQ GADYGQISEE TWTYLNSLYG GGPEIAIRQS VAQPLGPENL
901HGEQKIEAET RAV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

913 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.