Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
UNC84B / SUN2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113262-100
Catalog Number: LS-G113262
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SUN2 (NM_015374), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FRIGG | Nuclear envelope protein | Protein unc-84 homolog B | Sad1 unc-84 domain protein 2 | Sad1/unc-84 protein-like 2 | SUN2 | Unc-84 homolog B | Unc-84 homolog B (C. elegans) | KIAA0668 | Rab5-interacting protein | RAB5IP | UNC84B
- Origin Species
- Human
- NCBI GenBank
- NM_015374
- SwissProt
- Q9UH99
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSRRSQRLTR YSQGDDDGSS SSGGSSVAGS QSTLFKDSPL RTLKRKSSNM KRLSPAPQLG61PSSDAHTSYY SESLVHESWF PPRSSLEELH GDANWGEDLR VRRRRGTGGS ESSRASGLVG121RKATEDFLGS SSGYSSEDDY VGYSDVDQQS SSSRLRSAVS RAGSLLWMVA TSPGRLFRLL181YWWAGTTWYR LTTAASLLDV FVLTRRFSSL KTFLWFLLPL LLLTCLTYGA WYFYPYGLQT241FHPALVSWWA AKDSRRPDEG WEARDSSPHF QAEQRVMSRV HSLERRLEAL AAEFSSNWQK301EAMRLERLEL RQGAPGQGGG GGLSHEDTLA LLEGLVSRRE AALKEDFRRE TAARIQEELS361ALRAEHQQDS EDLFKKIVRA SQESEARIQQ LKSEWQSMTQ ESFQESSVKE LRRLEDQLAG421LQQELAALAL KQSSVAEEVG LLPQQIQAVR DDVESQFPAW ISQFLARGGG GRVGLLQREE481MQAQLRELES KILTHVAEMQ GKSAREAAAS LSLTLQKEGV IGVTEEQVHH IVKQALQRYS541EDRIGLADYA LESGGASVIS TRCSETYETK TALLSLFGIP LWYHSQSPRV ILQPDVHPGN601CWAFQGPQGF AVVRLSARIR PTAVTLEHVP KALSPNSTIS SAPKDFAIFG FDEDLQQEGT661LLGKFTYDQD GEPIQTFHFQ APTMATYQVV ELRILTNWGH PEYTCIYRFR VHGEPAHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
717 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.