LSBio
Human UNC84B / SUN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human UNC84B / SUN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SUN2 antibodies →
$220.00each
Catalog Number: LS-G92495-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SUN2 (NM_015374) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FRIGG | Nuclear envelope protein | Protein unc-84 homolog B | Sad1 unc-84 domain protein 2 | Sad1/unc-84 protein-like 2 | SUN2 | Unc-84 homolog B | Unc-84 homolog B (C. elegans) | KIAA0668 | Rab5-interacting protein | RAB5IP | UNC84B
Origin Species
Human
NCBI GenBank
NM_015374
SwissProt
Q9UH99

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSRRSQRLTR YSQGDDDGSS SSGGSSVAGS QSTLFKDSPL RTLKRKSSNM KRLSPAPQLG
61PSSDAHTSYY SESLVHESWF PPRSSLEELH GDANWGEDLR VRRRRGTGGS ESSRASGLVG
121RKATEDFLGS SSGYSSEDDY VGYSDVDQQS SSSRLRSAVS RAGSLLWMVA TSPGRLFRLL
181YWWAGTTWYR LTTAASLLDV FVLTRRFSSL KTFLWFLLPL LLLTCLTYGA WYFYPYGLQT
241FHPALVSWWA AKDSRRPDEG WEARDSSPHF QAEQRVMSRV HSLERRLEAL AAEFSSNWQK
301EAMRLERLEL RQGAPGQGGG GGLSHEDTLA LLEGLVSRRE AALKEDFRRE TAARIQEELS
361ALRAEHQQDS EDLFKKIVRA SQESEARIQQ LKSEWQSMTQ ESFQESSVKE LRRLEDQLAG
421LQQELAALAL KQSSVAEEVG LLPQQIQAVR DDVESQFPAW ISQFLARGGG GRVGLLQREE
481MQAQLRELES KILTHVAEMQ GKSAREAAAS LSLTLQKEGV IGVTEEQVHH IVKQALQRYS
541EDRIGLADYA LESGGASVIS TRCSETYETK TALLSLFGIP LWYHSQSPRV ILQPDVHPGN
601CWAFQGPQGF AVVRLSARIR PTAVTLEHVP KALSPNSTIS SAPKDFAIFG FDEDLQQEGT
661LLGKFTYDQD GEPIQTFHFQ APTMATYQVV ELRILTNWGH PEYTCIYRFR VHGEPAH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

717 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.