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UNC13A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UNC13A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all UNC13A antibodies →
$725.00each
Catalog Number: LS-G120210-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UNC13A (NM_001080421), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1032 | Munc13-1 | Unc-13 homolog A (C. elegans) | UNC13A | Protein unc-13 homolog A
Origin Species
Human
NCBI GenBank
AB028955
SwissProt
Q9UPW8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
202.12 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGGGGTCTIS VQVRVCTGGN LLGGRRRPLS PCNEEVWGEA PLPGARPFNA PPARSAPAPS
61GVPGLRLPAP HQRHSRPGRL RPTCELGDMS LLCVGVKKAK FDGAQEKFNT YVTLKVQNVK
121STTIAVRGSQ PSWEQDFMFE INRLDLGLTV EVWNKGLIWD TMVGTVWIPL RTIRQSNEEG
181PGEWLTLDSQ VIMADSEICG TKDPTFHRIL LDTRFELPLD IPEEEARYWA KKLEQLNAMR
241DQDEYSFQDE QDKPLPVPSN QCCNWNYFGW GEQHNDDPDS AVDDRDSDYR SETSNSIPPP
301YYTTSQPNAS VHQYSVRPPP LGSRESYSDS MHSYEEFSEP QALSPTGSSR YASSGELSQG
361SSQLSEDFDP DEHSLQGSDM EDERDRDSYH SCHSSVSYHK DSPRWDQDEE ELEEDLEDFL
421EEEELPEDEE ELEEEEEEVP DDLGSYAQRE DVAVAEPKDF KRISLPPAAP GKEDKAPVAP
481TEAPDMAKVA PKPATPDKVP AAEQIPEAEP PKDEESFRPR EDEEGQEGQD SMSRAKANWL
541RAFNKVRMQL QEARGEGEMS KSLWFKGGPG GGLIIIDSMP DIRKRKPIPL VSDLAMSLVQ
601SRKAGITSAL ASSTLNNEEL KNHVYKKTLQ ALIYPISCTT PHNFEVWTAT TPTYCYECEG
661LLWGIARQGM RCTECGVKCH EKCQDLLNAD CLQRAAEKSS KHGAEDRTQN IIMVLKDRMK
721IRERNKPEIF ELIQEIFAVT KTAHTQQMKA VKQSVLDGTS KWSAKISITV VCAQGLQAKD
781KTGSSDPYVT VQVGKTKKRT KTIYGNLNPV WEENFHFECH NSSDRIKVRV WDEDDDIKSR
841VKQRFKRESD DFLGQTIIEV RTLSGEMDVW YNLDKRTDKS AVSGAIRLHI SVEIKGEEKV
901APYHVQYTCL HENLFHFVTD VQNNGVVKIP DAKGDDAWKV YYDETAQEIV DEFAMRYGVE
961SIYQAMTHFA CLSSKYMCPG VPAVMSTLLA NINAYYAHTT ASTNVSASDR FAASNFGKER
1021FVKLLDQLHN SLRIDLSMYR NNFPASSPER LQDLKSTVDL LTSITFFRMK VQELQSPPRA
1081SQVVKDCVKA CLNSTYEYIF NNCHELYSRE YQTDPAKKGE VLPEEQGPSI KNLDFWSKLI
1141TLIVSIIEED KNSYTPCLNQ FPQELNVGKI SAEVMWNLFA QDMKYAMEEH DKHRLCKSAD
1201YMNLHFKVKW LYNEYVTELP AFKDRVPEYP AWFEPFVIQW LDENEEVSRD FLHGALERDK
1261KDGFQQTSEH ALFSCSVVDV FSQLNQSFEI IKKLECPDPQ IVGHYMRRFA KTISNVLLQY
1321ADIISKDFAS YCSKEKEKVP CILMNNTQQL RVQLEKMFEA MGGKELDAEA SDILKELQVK
1381LNNVLDELSR VFATSFQPHI EECVKQMGDI LSQVKGTGNV PASACSSVAQ DADNVLQPIM
1441DLLDSNLTLF AKICEKTVLK RVLKELWKLV MNTMEKTIVL PPLTDQTMIG NLLRKHGKGL
1501EKGRVKLPSH SDGTQMIFNA AKELGQLSKL KDHMVREEAK SLTPKQCAVV ELALDTIKQY
1561FHAGGVGLKK TFLEKSPDLQ SLRYALSLYT QATDLLIKTF VQTQSAQGLG VEDPVGEVSV
1621HVELFTHPGT GEHKVTVKVV AANDLKWQTS GIFRPFIEVN IIGPQLSDKK RKFATKSKNN
1681SWAPKYNESF QFTLSADAGP ECYELQVCVK DYCFAREDRT VGLAVLQLRE LAQRGSAACW
1741LPLGRRIHMD DTGLTVLRIL SQRSNDEVAK EFVKLKSDTR SAEEGGAAPA P
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1791 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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